Last update 8/9/2006

Eye Research Network Mitton Lab Research Tools Online Control of Gene Expression Laboratory Oakland University Eye Research Institute Rochester MI

These are useful internet resources that our laboratory uses on a daily basis. We use them for design and ordering of primers for PCR and PCR cloning, reading of our DNA sequencing reactions (when we are using dye-tagged), finding gene structures in human and mouse genome data that match our cDNAs etc. Our Yeast Two Hybrid protocols for transformation are based on Dan Gietz's lab protocols at the University of Manatoba (see list below). We typically get 1-5 million transformants per screen from 10-2- ng of library plasmid DNA. Thanks to Dan for advice in transforming yeast when I was a humble post doc at the Kellogg Eye Center (U. Mich.) starting off with yeast two-hyrbid methods for the first time.

• National Disease Research Interchange
• American Type Culture Collection
• Iowa State DNA Sequencing Facility

II. Genomic and Proteomic Tools

• NCBI TOOL ACCESS:Pubmed, Entrez, BLAST, OMIM, Taxonomy, Stucture
• ExPASy WWWeb Molecular Biology server - University of Geneva. (Swiss-Prot/EMBL/Pro-site/Tools)
• GeneScan Web Server at MIT....find those introns/exons in DNA
• NCBI ELECTRONIC PCR....find sequence markers in your DNA
• Marshfield Center for Medical Genetics....make genetic maps
• STS-Based Map of Human Genome....SEE your markers here
• Whitehead Institute forBiomedical Research/MIT Center for Genome
• UniGene: Non-redundant Organization of Genbank Sequences in Gene-Oriented Clusters. (Excellent!)
• T.I.G.R. Databases Access
• Order I.M.A.G.E. Clones from IMAGE  Consortium
• Mammalian Gene Collection-NIH Full Length Tissue Clone Resource
• Molecular Sequence Analysis Tools at Whitehead
• Primer3- primer design site
• Yeast Transformation- Gietz Lab, University of Manitoba

III. Large Indexes to Scientific Resources

• National Human Genome Research Institute-NIH
• NHGRI Research Resources Index (world)
• Harvard Biological Laboratories
• Stanford Genomic Resourses
• MedWeb: Biomedical Internet Resources (Emory)
• BioSCI News Groups